FastQCFastQC Report
Sat 31 Dec 2022
SRR1954853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177217
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19381.09357454420287No Hit
GAATCTATCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC11950.6743145409300463No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG11380.6421505837476088No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC9610.5422730324968823No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.3131753725658374No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG3770.21273354136454176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.207090741858851No Hit
GAATGAATGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT3380.1907266232923478No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC3360.18959806339120963No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC3310.18677666363836426No Hit
GCTGTCACTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC3310.18677666363836426TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTATTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT3130.1766196245281209TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC3040.1715411049729992No Hit
GAACTGACTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT2890.16307690571446307No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2640.14896990695023615No Hit
CCTGTCACTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC2290.1292201086803185TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.11059887031153895No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.1094703104104008No Hit
GAATGAAACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA1890.10664891065755543No Hit
AAATTTAGGTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAA1820.1026989510035719No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)