FastQCFastQC Report
Sat 31 Dec 2022
SRR1954852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732581
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.7769789279274237No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC29380.4010478022225528RNA PCR Primer, Index 11 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG24870.33948464401888667No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT23320.31832657412627413TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC22560.3079522946950576No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC21140.28856877259989Illumina PCR Primer Index 11 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC19510.26631867329346515RNA PCR Primer, Index 11 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC12340.16844553708054127Illumina PCR Primer Index 11 (100% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.13227206274801012No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC9250.12626590097204268No Hit
ACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC8880.12121526493316097TruSeq Adapter, Index 11 (96% over 26bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT7400.10101272077763415TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)