FastQCFastQC Report
Sat 31 Dec 2022
SRR1954851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290828
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.5580618097294621No Hit
GAATCTATCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC7950.2733574483887384TruSeq Adapter, Index 11 (95% over 23bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG6150.21146519592336366No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC5860.20149366635949773No Hit
CTGTCTATTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT5290.1818944530787957TruSeq Adapter, Index 11 (96% over 27bp)
GCTGTCACTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC4990.17157907766789993Illumina PCR Primer Index 11 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.15335524777531737No Hit
CCTGTCACTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC4350.1495729434579889RNA PCR Primer, Index 11 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.11415682121391339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)