FastQCFastQC Report
Sat 31 Dec 2022
SRR1954850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762301
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56880.7461619491513195No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC46940.6157672625380264No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC45760.6002878128193456No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG32270.42332359527273344No Hit
TGAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT23640.3101137214827214No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC22440.29437190820948683No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT22310.2926665451048864TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC22030.28899345534113163TruSeq Adapter, Index 16 (95% over 21bp)
TGAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC18130.23783256220311924No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC14800.19414903036989326TruSeq Adapter, Index 16 (95% over 21bp)
TGAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATG14750.19349312148350847No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT13470.17670185399205826No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC12200.16004176827788497TruSeq Adapter, Index 16 (95% over 21bp)
TGAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGT11310.14836659010023598No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC10600.1390526839135722No Hit
TGAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC10090.1323624132724475No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT9890.1297387777269084No Hit
TGAATGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT9720.1275086875132002No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.12580332440859976No Hit
TGAAAGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC8990.11793241777198246No Hit
TTGTACGGGAACTTAGTCTTTTGGCAAATGACAGATTGATGACCATAAACC8560.1122916013490734No Hit
TGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTG8410.11032387468991908No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT7920.10389596760334828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)