FastQCFastQC Report
Sat 31 Dec 2022
SRR1954849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299439
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.5319948303327221No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC12260.40943230507716094No Hit
GAATCTATCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC11070.36969132277358663No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG7080.2364421468145432No Hit
CTGTCTATTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT5050.16864870641432814TruSeq Adapter, Index 13 (95% over 22bp)
TGAATCAGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT5040.1683147485798443No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.16163559189016793No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC4840.16163559189016793No Hit
GCTGTCACTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC4800.1602997605522327TruSeq Adapter, Index 16 (95% over 21bp)
TGAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATG4020.1342510494624949No Hit
TGAACTATCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC3550.11855503124175541No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3410.11387962155898197No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC3170.10586463353137034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)