FastQCFastQC Report
Sat 31 Dec 2022
SRR1954844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640908
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC107170.6531140076104206RNA PCR Primer, Index 29 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90800.5533521684335746No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG82850.5049033827612517No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC61660.3757675628371609No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC47410.288925399839601Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT36720.2237785421242385RNA PCR Primer, Index 29 (96% over 26bp)
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT33200.20232700431712197TruSeq Adapter, Index 12 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC26700.16271478961648062RNA PCR Primer, Index 29 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC25530.1555845909703652No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT24130.14705272934253474No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCT22530.13730203033929994No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTA19370.11804439980791125No Hit
GAATATGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC17350.10573414231632729RNA PCR Primer, Index 29 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC16860.10274799074658665No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC16480.10043219973331838Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)