FastQCFastQC Report
Sat 31 Dec 2022
SRR1954843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638876
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.4055560077385909No Hit
GAATCTATCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC25490.398981962070887No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG18630.29160588283172323No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC13270.2077085381200734No Hit
GCTGTCACTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC9220.14431595489578572Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTATTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT7510.11755019753441982TruSeq Adapter, Index 12 (95% over 24bp)
GAACTGACTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT6440.10080203357145988RNA PCR Primer, Index 29 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)