FastQCFastQC Report
Sat 31 Dec 2022
SRR1954842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940963
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73180.7777138952328625No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC71040.7549712369136725No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG70970.7542273181836056No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC48440.5147917612063386No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC23990.25495157620437786No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT21330.22668266446183327Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT19960.21212311217337984No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCTTGTT19860.21106037113042703No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCT18700.19873257503217448No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTC17850.18969927616707566No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTA16320.17343933820989774No Hit
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC15840.16833818120372426No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.133374000890577No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG12160.12922931082306105No Hit
GAATATGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC10580.11243800234440673No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC10330.10978114973702474No Hit
TCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC9450.10042902855904004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)