FastQCFastQC Report
Sat 31 Dec 2022
SRR1954840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993294
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62250.6267026680922265No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC50480.5082080431372785No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG41000.4127680223579323No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC29040.2923605699823013Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC27840.28027955469377647No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT27460.27645389985241026Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC20270.20406848324866556Illumina PCR Primer Index 11 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT15010.15111336623396499Illumina PCR Primer Index 11 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC13400.1349046707218608Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC12420.12503850823623217No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT11990.1207094777578441No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.11295749294770732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)