FastQCFastQC Report
Sat 31 Dec 2022
SRR1954839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384362
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.460243208225579No Hit
GAATCTATCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC12730.331198193369792No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG9170.23857717464265457No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC6430.16729021079086903No Hit
GCTGTCACTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC6330.16468849678167977Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTATTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT5710.14855786992470638Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCACTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC4260.11083301679146221Illumina PCR Primer Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)