FastQCFastQC Report
Sat 31 Dec 2022
SRR1954838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675130
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82761.225837986758106No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTC42500.6295083909765526No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCG37630.55737413535171No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.19966524965562188No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGC13240.19611037874187193No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT13040.19314798631374697No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT11460.16974508613155986No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTC11080.16411654051812244No Hit
CCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC9640.14278731503562278TruSeq Adapter, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7420.10990475908343579No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCC7390.10946040021921703No Hit
GCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC6780.10042510331343593TruSeq Adapter, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)