FastQCFastQC Report
Sat 31 Dec 2022
SRR1954836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450299
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.7943610800823453No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG22900.5085509850121808No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC22320.4956706543874182No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC17370.3857436947450472No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC8250.1832115994039516Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT6850.15212114617176586Illumina Single End Adapter 2 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.15123284750798913No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGCAGGT6560.14568098085938455No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT5900.13102405290706842No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC5710.12680463425412894Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT5480.12169691693741269No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTC5390.11969824494391505No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5170.114812602293143No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC4680.103930943661878No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTA4550.10104397300460362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)