FastQCFastQC Report
Sat 31 Dec 2022
SRR1954833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335871
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC16560.49304643747152926No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG14990.4463023005856415No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.3623414942046202No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC11070.3295908250489027No Hit
GCTGTCACTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC6910.20573374896909824TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTATTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT5620.16732614605012044Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC4330.1289185431311426No Hit
CCTGTCACTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC4080.12147520923211591TruSeq Adapter, Index 14 (95% over 21bp)
GAATGAATGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT3790.11284094190924493No Hit
GAACTGACTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT3720.11075680841751744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)