FastQCFastQC Report
Sat 31 Dec 2022
SRR1954828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709204
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51830.7308193411204674No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT15840.2233489940835077Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC12740.17963801670605356Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC12350.17413889374566416Illumina Single End Adapter 2 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.131273935285193No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC8760.12351876187951562No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8230.11604559477949927No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC8110.11435355694553329Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC8030.11322553172288934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)