FastQCFastQC Report
Sat 31 Dec 2022
SRR1954827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289997
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.5375917681907054No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.14793256481963607No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4230.14586357789908172No Hit
CTGTCTATTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT3620.12482887754011247Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCACTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC2940.10138035910716317Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)