FastQCFastQC Report
Sat 31 Dec 2022
SRR1954822.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954822.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841514
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC244541.3279290844381308No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG191551.040176724151975No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC173090.9399331202477962No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC126980.6895413230635228TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT122460.6649963019558907TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106040.5758305394365724No Hit
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC91640.4976340120140276TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT79050.42926635366334437No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT61410.3334756075707271No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC61000.33124917866494635No Hit
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC48760.26478213035578335TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCCCCT47520.25804854049439757No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTA38710.21020747059213232No Hit
ACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC35710.1939165273791022TruSeq Adapter, Index 19 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG34520.18745445323793355No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC33340.1810466822408084No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG25230.13700683242158354No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT22010.11952122003959784No Hit
AGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG18480.10035221019226571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)