FastQCFastQC Report
Sat 31 Dec 2022
SRR1954821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657275
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC52370.7967745616370622No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG38330.5831653417519302No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC38230.5816439085618652No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28070.4270662964512571No Hit
CTGTCTATTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT23560.35844965957932373TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCACTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC22480.34201818112662125TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCACTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC18020.27416226084972045TruSeq Adapter, Index 16 (95% over 22bp)
GAATGAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC14720.22395496557757405No Hit
GAACTGACTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT14240.2166520862652619No Hit
GAATGAATGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT13010.19793845802746185No Hit
TCTGTCACTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC9030.13738541706287324TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC8000.1217146552052033TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)