FastQCFastQC Report
Sat 31 Dec 2022
SRR1954820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945005
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66470.703382521785599No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT29190.30888725456479066Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC23180.24528970746186526No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC22440.237459061063169No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC21380.22624218919476616No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG18690.1977767313400458No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC15370.1626446420918408No Hit
TCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC13900.1470891688403765No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCC12260.12973476330813064No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.1216924778175777No Hit
ACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC10170.10761847820911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)