FastQCFastQC Report
Sat 31 Dec 2022
SRR1954814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585937
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65951.125547627133975No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT14280.2437122079677508No Hit
CCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC13970.23842153678637806No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.19387749877546562No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC11260.19217083065244217No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC8250.14080012014943585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7870.13431478128194668No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC7780.13277877997122559No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7350.1254401070422247No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG6740.11502943149178155No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC5990.10222942056910556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)