FastQCFastQC Report
Sat 31 Dec 2022
SRR1954812.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954812.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775056
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.7115614871699594No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT11900.1535372927891662TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC9500.12257178836109907TruSeq Adapter, Index 16 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.11831403150223985No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC9020.11637868747548565TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC7990.10308932515844017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)