FastQCFastQC Report
Sat 31 Dec 2022
SRR1954807.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954807.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665523
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31490.4731617089116379No Hit
GAATCTATCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC18430.27692506494891983No Hit
CTGTCTATTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT10010.15040802496683062Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC8240.12381240017249591No Hit
GCTGTCACTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC8080.12140827589730181No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG7960.11960518269090625No Hit

[FAIL]Adapter Content

Adapter graph