FastQCFastQC Report
Sat 31 Dec 2022
SRR1954806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005240
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.6500935100075603No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC24940.24809995622935818TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT24930.24800047749791096TruSeq Adapter, Index 22 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC24210.24083800883371137No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCG24030.23904739166766145No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGC16730.16642791771119333No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC15920.1583701404639688TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC13770.13698221320281723TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC12270.12206040348573474No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.11509689228442958No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC10590.10534797660260237TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)