FastQCFastQC Report
Sat 31 Dec 2022
SRR1954805.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954805.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393801
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.4522588820241696No Hit
CTGTCTATTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT4620.11731813784119391TruSeq Adapter, Index 22 (95% over 22bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCG4460.11325517202851185No Hit
GAATCTATCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC4450.11300123666521925No Hit
CCTGTCACTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC4310.10944614157912246TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)