FastQCFastQC Report
Sat 31 Dec 2022
SRR1954802.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954802.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459892
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74611.6223374183503954No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.28963321823384625No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT8150.1772155201656041Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCC6950.15112243744183415No Hit
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC6710.14590382089708018No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC6700.14568637854104877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5870.12763866299044122No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4730.10285023440285981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)