FastQCFastQC Report
Sat 31 Dec 2022
SRR1954800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287077
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133171.034670031396723No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC19700.15305999563351688No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCC17290.13433539718291912No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG16560.12866363084726087No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT16330.12687663597438226Illumina PCR Primer Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC15550.12081639249244605Illumina PCR Primer Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)