FastQCFastQC Report
Sat 31 Dec 2022
SRR1954796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466837
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73901.582993635894327No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT17980.3851451363109608Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC14690.3146708594220253No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC14660.31402823683641184No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG12310.2636894676300293No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.25319329873167723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11220.24034084701940933No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC9990.2139933210092602No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC8440.18079115408590152No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC7830.16772449484509583No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC7440.1593704012321217No Hit
ACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC5430.1163146879960243No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)