FastQCFastQC Report
Sat 31 Dec 2022
SRR1954790.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954790.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424387
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62391.4701204325297428No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG25460.5999241258568241No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC24740.5829584789354999No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC22830.5379523877969872No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT14270.33624969662124427TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC13380.3152782719546075TruSeq Adapter, Index 15 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.26320316126554305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10700.2521283639696786No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC9070.21372002441168084No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC8910.20994988065138662TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATAGTGT7910.18638648214954748No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA7350.17319097898851754No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT7150.16847829928814975No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT6640.15646096605221177No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC5910.13925968514586923No Hit
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC5410.12747798589494966TruSeq Adapter, Index 15 (95% over 22bp)
TTAGTAGGGATTTAGATTTTGATGAGATTCAGGAAATTATTGAAAGTTTGG4780.11263304483879102No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4310.10155824754292661No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG4270.10061571160285304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)