FastQCFastQC Report
Sat 31 Dec 2022
SRR1954789.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954789.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166025
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17691.0655021834061136No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC6320.3806655624152989No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG6070.3656075892184912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.3192290317723234No Hit
GAATCTATCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC5270.31742207498870656No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.2788736636048788No Hit
CTGTCTATTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT3220.1939466947748833TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCACTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC2690.16202379159765096TruSeq Adapter, Index 15 (95% over 22bp)
TTAGTAAGGATTTAGATTTTGATGAGATTCAGGAAATTATTGAAAGTTTGG2170.13070320734829094No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1850.11142900165637705No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC1830.11022436380063244No Hit
CCTGTCACTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC1810.10901972594488782TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG1680.1011895798825478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)