FastQCFastQC Report
Sat 31 Dec 2022
SRR1954779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215815
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27891.2923105437527511No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.36327410050274545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7280.337325950466835No Hit
CTGTCTATTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT4780.22148599494937793Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC3070.1422514653754373No Hit
GCTGTCACTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC3050.14132474573129764No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.11583995551745709No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.11213307694089844No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.10379260014364154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)