FastQCFastQC Report
Sat 31 Dec 2022
SRR1954778.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954778.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299437
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44711.4931354508627859No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG17780.593780995668538No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC16030.5353379842838393No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC12760.42613304301071675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10010.3342940251204761No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC8480.2831981351669967TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT8050.2688378523696136TruSeq Adapter, Index 16 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.25848509035289563No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC7200.24045124683990288TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC6770.2260909640425198No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT5170.17265735363365248No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT4920.16430835200726696No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT4410.1472763886894405No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA3950.13191422569689115No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC3810.12723878478611528TruSeq Adapter, Index 13 (95% over 21bp)
TCGTAAGGGTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCC3570.11922374322478517No Hit
ACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC3420.11421434224895388TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC3300.11020682146828883No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3280.10953890133817798No Hit
TGATAAGGGGATTTATACATATTTTAGATAAAAAAAGTTAAATGCTCTTAT3120.10419554029729124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)