FastQCFastQC Report
Sat 31 Dec 2022
SRR1954777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118683
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12061.0161522711761584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.3968554889916837No Hit
GAATCTATCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC4400.37073548865465145No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG3980.33534710110125293No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.2923754876435547No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC2910.24519097090568995No Hit
CTGTCTATTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT2120.17862709907905935TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCACTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC1750.14745161480582727TruSeq Adapter, Index 13 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1640.138183227589461No Hit
GCTGTCACTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC1600.1348129049653278TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG1350.11374838856449533No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.11206322725242873No Hit
TGATAAAGGGATTTATACATATTTTAGATAAAAAAAGTTAAATGCTCTTAT1190.10026709806796257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)