FastQCFastQC Report
Sat 31 Dec 2022
SRR1954775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334652
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.6391714377920945No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.1858647191709597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.12819286901019566No Hit
CTGTCTATTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT4190.12520469024538924TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)