FastQCFastQC Report
Sat 31 Dec 2022
SRR1954772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541749
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176131.142403854323888No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC74420.4826985456128073No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT34080.22104765431986662No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT33900.21988014910338843RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC32020.20768620573128313RNA PCR Primer, Index 16 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT29900.1939355887372069No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT29800.19328697472805234RNA PCR Primer, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG29760.19302752912439056No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC28280.1834280417889034No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG27030.17532036667447165RNA PCR Primer, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC25350.1644236513206754No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC23910.15508360958885006RNA PCR Primer, Index 14 (95% over 23bp)
GTGAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT23420.15190540094399282No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC21620.14023034877921114No Hit
CGTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT17660.11454523401669144RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT17030.11045896575901785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)