FastQCFastQC Report
Sat 31 Dec 2022
SRR1954768.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954768.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579160
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50890.8786863733683266No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT15530.26814697147593064Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC12570.2170384695075627No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC11680.20167138614545205No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.15505214448511637No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG8620.14883624559707162No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC8330.14382899371503557No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC7770.13415981766696594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.11257683541681054No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC5910.10204434007873471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)