FastQCFastQC Report
Sat 31 Dec 2022
SRR1954766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612820
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62881.02607617244868No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG50930.831076009268627No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC45810.7475278221990145No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC25030.40843967233445383No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC22720.37074508012140595TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT22510.3673182990111289TruSeq Adapter, Index 16 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT16770.2736529486635554No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC15250.24884958062726414TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT14900.24313827877680233No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT14500.23661107666198886No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC11580.1889625012238504No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.17297085604255735No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA9860.1608955321301524No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG9010.14702522763617376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8640.14098756567997128No Hit
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC8160.1331549231421951TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC7910.1290754218204367No Hit
ACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC7080.11553147743219869TruSeq Adapter, Index 16 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC6550.10688293463007083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)