FastQCFastQC Report
Sat 31 Dec 2022
SRR1954765.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954765.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235111
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.7494332464240295No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG12020.5112478786615683No Hit
GAATCTATCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC10810.45978282598432235No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC5960.2534972842614765No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.21266550693076888No Hit
CTGTCTATTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT4860.20671087273670735TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCACTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC4590.1952269353624458TruSeq Adapter, Index 19 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.1688564125030305No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG3770.16034979222579973No Hit
GAACTGACTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT3600.1531191649901536No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC3540.15056717890698437No Hit
CCTGTCACTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC3170.13482993139410746TruSeq Adapter, Index 19 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC3080.13100195226935363No Hit
GAATGAATGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT3000.12759930415846132No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2590.11016073259013828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)