FastQCFastQC Report
Sat 31 Dec 2022
SRR1954764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875610
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC51900.5927296399081783No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC46880.5353981795548246No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG46010.525462249174861No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44810.5117575176162904No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC23040.26313084592455543No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT16520.18866847112298857Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT15400.1758773883349893No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGATCGTT14940.17062390790420393No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT12200.1393314375121344No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC12100.1381893765489202No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA11410.13030915590274209No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC10700.12220052306392115No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCC9720.11100832562442184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)