FastQCFastQC Report
Sat 31 Dec 2022
SRR1954762.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954762.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394461
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60821.5418507786574591No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG54661.3856883189973155No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC42851.0862924344865525No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC39961.0130279038992447No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT13920.35288659715409126No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC13100.3320987372642669No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGAT12430.31511353467136166No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT11970.3034520522941432No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC11590.29381865380861477No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.2887484440793894No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTA11200.2839317448366252No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG9770.2476797452726632No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT8560.21700497641084926Illumina Single End Adapter 2 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC6400.1622467113352144No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC6240.158190543551834No Hit
GAATATGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC6220.15768352257891147No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC5140.13030439004109406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4990.12650173274417498No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGT4980.1262482222577137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)