FastQCFastQC Report
Sat 31 Dec 2022
SRR1954760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787314
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111531.4165885529788622No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC49300.6261796436999723No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG42150.5353645432444997No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC30600.38866322712412077No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT27300.3467485653754411Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC23350.2965779854035366Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.2387865578409631No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC18800.2387865578409631Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT16520.20982733699642075No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC15790.20055530576110675No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT13630.17312025443469822No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC13440.17070698603098636No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC11950.15178188118082492Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTA11610.14746340087944582No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT11480.14581221723480087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10150.12891933840881784No Hit
ACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC8530.10834304991401145Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)