FastQCFastQC Report
Sat 31 Dec 2022
SRR1954759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298295
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29580.9916357967783569No Hit
GAATCTATCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC12300.4123434854757874No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG9740.32652240231985113No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.2698670778926901No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC7360.24673561407331668No Hit
CTGTCTATTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT6120.20516602691966007Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCACTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC5470.18337551752459813Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.16393167837208134No Hit
CCTGTCACTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC4250.14247640758309726Illumina Single End Adapter 1 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC3710.12437352285489198No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC3550.11900970515764595No Hit
GAACTGACTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT3540.11867446655156808No Hit
GAATGAATGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT3120.1045944450962973No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG3000.10057158182336277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)