FastQCFastQC Report
Sat 31 Dec 2022
SRR1954756.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954756.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445288
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68931.5479869208242756No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG22080.49585885988394024No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC21650.4862021882467077RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC15040.3377589335441333No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.2762257235766515No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT11420.2564632327841756RNA PCR Primer, Index 38 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC10220.2295143817035267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10050.22569662780043478No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC9360.21020103842906163RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC8090.18168017103537484RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT6490.14574836959450962RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC6260.14058317313738525No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT6130.13766371427031493No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC5910.13272309157219597RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT5510.12374014121197965No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA5260.11812579723684447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)