FastQCFastQC Report
Sat 31 Dec 2022
SRR1954755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178574
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18781.0516648560260733No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.30239564550270476No Hit
GAATCTATCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC5210.2917557987164985No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG4960.2777560003135955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.275516032569131No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC3670.20551704055461603No Hit
CTGTCTATTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT2610.1461578953263073RNA PCR Primer, Index 27 (95% over 24bp)
CCTGTCACTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC2100.11759830658438519RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.10919842754264339No Hit
GCTGTCACTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC1910.1069584597981789RNA PCR Primer, Index 27 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1041585001175983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)