FastQCFastQC Report
Thu 19 Jan 2023
SRR1954754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527286
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59391.1263337164271383No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.18566774008792192No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC8040.15247892035821167No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT7980.15134101796747873TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7000.132755278918841No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC5830.11056618229954901TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC5360.10165261357214112No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC5290.1003250607829527TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG302.1632568E-645.0000041
TAGTACG302.1632568E-645.0000041
GGCGTTG207.029811E-445.021
GACGTAA207.029811E-445.020
ATTACGG351.2102464E-745.02
ATAGCGG253.8879232E-545.02
ACGGGTA207.029811E-445.05
ACGGGCG800.045.05
GACGGTC207.029811E-445.029
TTGCGAG406.8030204E-945.01
CGCAGTA406.8030204E-945.037
TTAACGG351.2102464E-745.02
GCGGCGA207.029811E-445.09
GTATACG207.029811E-445.01
ACACTCG207.029811E-445.044
GCGTGAC207.029811E-445.031
TAGTGCG253.8879232E-545.01
AGTACGG453.8380676E-1045.02
AGTAACG253.8879232E-545.01
ATCGAAG253.8879232E-545.01