FastQCFastQC Report
Sat 31 Dec 2022
SRR1954752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395641
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39020.9862476335870144No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.17566430172808176No Hit
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT6550.16555412608905548RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC5530.13977317820953844Illumina PCR Primer Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC5070.12814647622465822RNA PCR Primer, Index 41 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.1218276164502668No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCC4620.11677252863075363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)