FastQCFastQC Report
Sat 31 Dec 2022
SRR1954748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424079
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64841.5289604059620967No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC10790.25443372579165674No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.25349050530679423No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG10260.24193605436722876No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT7990.1884082918512824TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.16860066166917012No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC7130.1681290514267389No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC6630.15633879536595777TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC6500.15327332879015468No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC5400.12733476545643618TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)