FastQCFastQC Report
Sat 31 Dec 2022
SRR1954740.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954740.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436726
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47941.097713440463815No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG25340.5802265035743235No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC25150.5758759496801198No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC19600.4487939806652226No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC13990.32033815252584No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT13780.3155296455901412Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.20584989215205873No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC8890.2035601269445831No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT8330.1907374417827196No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.19050846526197204No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCTGTT8260.18913460613748667No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT7420.16990057839469141No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC7290.1669238836249731No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC6840.15661994019133277No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA6710.15364324542161445No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC5300.12135755599620814No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG4740.10853487083434465No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC4500.10303943433640314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)