FastQCFastQC Report
Sat 31 Dec 2022
SRR1954739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169600
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.7523584905660378No Hit
GAATCTATCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC6120.3608490566037736No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG5440.32075471698113206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.27181603773584906No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC4400.25943396226415094No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.23172169811320753No Hit
CTGTCTATTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT3270.1928066037735849Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC2480.14622641509433962No Hit
CCTGTCACTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC1980.11674528301886794No Hit
GAACTGACTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT1900.11202830188679246No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC1820.10731132075471699No Hit
GAATGAATGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT1810.10672169811320754No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC1730.10200471698113206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)