FastQCFastQC Report
Sat 31 Dec 2022
SRR1954738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068768
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126711.1855706757687356No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC38460.35985358843079135Illumina PCR Primer Index 7 (95% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.20444100122758166No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG19810.18535360340129944No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC17360.16243001287463696No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC13900.13005628911045242No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC13850.12958846073235725Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT13280.12425521722207251Illumina PCR Primer Index 7 (96% over 28bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT12650.11836057965807359Illumina PCR Primer Index 7 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC12430.11630213479445492Illumina PCR Primer Index 7 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10870.10170588939788616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)