FastQCFastQC Report
Sat 31 Dec 2022
SRR1954737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439082
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.8203479076801145No Hit
GAATCTATCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC9720.22137095121184652Illumina PCR Primer Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4990.11364619820443563No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG4700.10704150933083115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)