FastQCFastQC Report
Sat 31 Dec 2022
SRR1954733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336502
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.6121806111107809No Hit
GAATCTATCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC11470.3408597868660513No Hit
CTGTCTATTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT8080.240117443581316Illumina Single End Adapter 2 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG7200.21396603883483606No Hit
GCTGTCACTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC6000.17830503236236336No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC5680.16879543063637065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.15037057729225978No Hit
GGGCTTAACTTTCTCATGAATTTGCCTGAGGTTTCCAAACCCTTCACATTT4730.14056380051232978No Hit
CCTGTCACTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC4400.1307570237323998No Hit
GAACTGACTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT4030.11976154673672074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)